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Comparison of Seeplex PneumoBacter Detection ACE assay with in-house multiplex PCR for identification of Streptococcus pneumonia

Luznik D, Kosnik M, Tomic V Published January 2015 Link to publication: http://www.ncbi.nlm.nih.gov/pubmed/25742147 Abstract Rapid and accurate identification of Streptococcus pneumoniae is important for appropriate and prudent antimicrobial use in the treatment of lower respiratory tract infection. It is difficult to separate S. pneumoniae from commensal viridans group streptococci either by classical techniques or molecular …

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A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods

Gavin J. Nixon, Helle F. Svenstrup,  Carol E. Donald, Caroline Carder, Judith M. Stephenson, Stephen Morris-Jones, Jim F. Huggett, Carole A. Foy Biomolecular Detection and Quantification, Vol 2, December 2014, Pages 4–10 Published December 2014 Link to publication: http://www.sciencedirect.com/science/article/pii/S2214753514000096 Abstract Molecular diagnostic measurements are currently underpinned by the polymerase chain reaction (PCR). There are also …

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Survey results on nucleic acid tests of infectious diseases: Present status and need for rapid and patient near diagnostics

Jörg Neukammer, Martin Hussels, A. Kummrow, Alison Devonshire, Carole Foy, Jim Huggett, Helen Parkes, Jana Zel, Mojca Milavec, Heinz Schimmel, Wolfgang Unger, Müslüm Akgoz, Timothy Mchugh, Viktorija Tomic, Hans-Peter Grunert, Heinz Zeichhardt Published February 2015 DOI: 10.3205/lab000016 Link to publication: http://www.egms.de/static/en/journals/lab/2015-6/lab000016.shtml Abstract This survey discusses current and emerging isothermal and rapid polymerase chain reaction (PCR) …

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Standardising clinical measurements of bacteria and viruses using nucleic acid tests

Pavšič J, Devonshire AS, Parkes H, Schimmel H, Foy CA, Karczmarczyk M, Gutiérrez-Aguirre I, Honeyborne I, Huggett JF, McHugh TD, Milavec M, Zeichhardt H, Zel J. J Clin Microbiol. 2014 Nov 12. pii: JCM.02136-14. [Epub ahead of print] Published November 2014 DOI: 10.1128/JCM.02136-14 Link to publication: http://www.ncbi.nlm.nih.gov/pubmed/25392365 Abstract Nucleic acid based tests for infectious diseases …

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Highly Reproducible Absolute Quantification of Mycobacterium tuberculosis Complex by Digital PCR

Alison S. Devonshire , Isobella Honeyborne , Alice Gutteridge , Alexandra S. Whale , Gavin Nixon , Philip Wilson, Gerwyn Jones , Timothy D. McHugh , Carole A. Foy , and Jim F. Huggett Anal. Chem., Article ASAP Published February 2015 DOI: 10.1021/ac5041617 Link to publication: http://pubs.acs.org/doi/abs/10.1021/ac5041617?journalCode=ancham Abstract Digital PCR (dPCR) offers absolute quantification through …

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Molecular Diagnostics: Current Research and Applications (Book)

Publisher: Caister Academic Press Editor: Jim Huggett and Justin O’Grady Hardback: Publication date: May 2014 Ebook: Publication date: April 2014 Link to publication: http://www.horizonpress.com/moleculardiagnostics Abstract The application of molecular technology in clinical diagnosis is a rapidly developing area and is predicted to greatly improve the speed, efficiency and accuracy of diagnostic medicine. The editors of …

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The Molecular Bacterial Load Assay Replaces Solid Culture for Measuring Early Bactericidal Response to Antituberculosis Treatment

Isobella Honeyborne, Bariki Mtafya, Patrick P. J. Phillips, Michael Hoelscher, Elias N. Ntinginya, Anke Kohlenberg, Andrea Rachow, Gabriel Rojas-Ponce, Timothy D. McHugh and Norbert Heinrich on behalf of the Pan African Consortium for the Evaluation of Anti-tuberculosis Antibiotics (PanACEA) J. Clin. Microbiol. August 2014 vol. 52 no. 8 3064-3067 Published May 2014 DOI: 10.1128/JCM.01128-14 Link to …

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Comparative Study of Sensitivity, Linearity, and Resistance to Inhibition of Digital and Nondigital Polymerase Chain Reaction and Loop Mediated Isothermal Amplification Assays for Quantification of Human Cytomegalovirus

Anal. Chem., 2014, 86 (9), pp 4387–4394 Gavin Nixon, Jeremy A Garson, Paul Grant, Eleni Nastouli, Carole A. Foy, Jim F. Huggett Published March 2014 DOI:10.1021/ac500208w Link to publication: http://pubs.acs.org/doi/abs/10.1021/ac500208w Abstract Performing nucleic acid amplification techniques (NAATs) in digital format using limiting dilution provides potential advantages that have recently been demonstrated with digital polymerase chain …

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Deliverable D3.1.3

Technology scoped and technologies / instrumentation for assessment identified. HLT08 INFECT-MET D3.1.3

Deliverable D1.6.3

Status report and evaluation of the potential applications of single molecule detection schemes for Tasks 1.7 and 1.8 and WP3 and WP4 HLT08 INFECT_MET D1.6.3

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